Expression levels of matrix metalloproteinase 2 protein in preinvasive and invasive cervical lesions and its clinicopathological association: a North Indian study

Rekha Sachan, Departments of Obstetrics & Gynaecology, King George Medical University, LucknowFollow
Pushp L. Sachan, Department of Physiology, Autonomous State Medical College, Hardoi, Uttar Pradesh, India
Ayushi Shukla, Departments of Obstetrics & Gynaecology, King George Medical University, Lucknow
Malti Maurya, Departments of Pathology, King George Medical University, Lucknow
Munna L. Patel, Department of Medicine, King George Medical University, Lucknow

Article Type

Original Study

Subject Area

Obstetrics and Gynecology

Abstract

Background Matrix metalloproteinase (MMPs) play an important role in development of female genital cancers and metastasis. The aim of this study was to analyze expression levels of MMP2 in various grades of cervical intraepithelial neoplasia (CIN) and squamous cell carcinoma (SCC) of cervix and its association with clinicopathological variables. Patients and methods This prospective study was carried out in a tertiary care center in Northern India over a period of 1 year. A total of 664 participants with gynecological complaints and unhealthy cervix were recruited for colposcopy. Of these, 70 biopsy samples were obtained from higher grade colposcopy lesions, where 15 were of chronic cervicitis, 40 samples of CIN [CIN1 (14), CIN2 (eight), and CIN3 (18)], and 15 cases of SCC of cervix. All samples were enrolled for final analysis. Results Positive association of MMP2 expression was found with advanced age (46–55 years) and high parity (>5). Based on clinicopathological aspects, MMP2 expression had no association or 0 score with discharge per vaginum. On the contrary, postcoital bleeding even less than 6 months had a strong positive MMP2 expression score. Postmenopausal bleeding also showed positive association with MMP2 expression. Overall, 93.3% samples of chronic cervicitis were negative for MMP2. Moreover, 7.1% CIN1 biopsy samples showed MMP2 positive expression, 37.5% of CIN2 samples, and 50% of CIN3 samples showed MMP2 positive expressions, where 33.3% were strongly positive. MMP2 expression was positive in 80% of biopsy samples of the cancer of cervix, being strongly positive (score=3) in 66.67%. Overall positive MMP2 expression in CIN was 32.5% and in SCC was 80%. As disease severity increased, the expression of MMP2 also increased. Conclusions In CIN lesions, MMP2 positive expressions and intensity of expression increased with increase in severity of lesions, which reached the maximum in invasive cancer.

IRB Number

0th ECM II B-Thesis/P40

Keywords

cervical cancer, cervical intraepithelial neoplasia, clinicopathological association, immunohistochemistry, matrix metalloproteinase 2 protein expression

Recommended Citation

Sachan, Rekha; Sachan, Pushp L.; Shukla, Ayushi; Maurya, Malti; and Patel, Munna L. (2023) "Expression levels of matrix metalloproteinase 2 protein in preinvasive and invasive cervical lesions and its clinicopathological association: a North Indian study," Journal of Medicine in Scientific Research: Vol. 6: Iss. 1, Article 5.
DOI: https://doi.org/10.59299/2537-0928.1023