•  
  •  
 

Article Type

Original Study

Abstract

Background Nephrolithiasis remains a public health problem around the world, affecting up to 20% of the population. There are many risk factors that increase one's susceptibility to kidney stone formation. These include specific food, urinary tract infection, kidney disorders, and metabolic disorder. Also, some people with a family history of kidney stones may be more susceptible to develop stones. Objective The aim was to isolate, purify, and identify bacteria from urinary stones. Also, determine the most bacterial isolate secreting uricase enzyme. Patients and methods A total of 100 cases were included in the study (66 men, 34 women) at the National Institute of Urology and Nephrology, Nephrology Department. Urine and stone culture identification of bacteria and purification of uricase enzyme. Results The study showed that there was the highest percentage of stones within the greater than 40 years age group and uric acid stones constitute the highest incidence (40%). The highest percentage of bacterial isolates was Klebsiella spp. (25%). Klebsiella pneumonia was the most potent uricase producer, out of all bacterial species isolated from human urine. Conclusion The study reported that uric acid stones were significantly affected by the uricase enzyme.

Keywords

Uricase enzyme, bacterial species, kidney stones, Klebsiella pneumonia

Download

Share

COinS